Sap abap table plko task list header sap datasheet. Mtr4 drives liver tumorigenesis by promoting cancer. Getting started with shrna in the pgipz lentiviral vector. Novartis plkoteton the allinone system for the inducible expression of shrna dmitri wiederschain, ph. There is a little that i dont understand regarding the age1. I have a question about the data i see in files mapl and plko and whats displayed via the ca03 tcode. Mix by pipetting gently, incubate at room temperature for 1 hr, and then ethanol precipitate and. Addgene is working with the trc to make this shrna cloning vector available to the scientific community. Ideal for recombinant lentivirus production trans lenti.
The protocol below is optimized for transfection of the shrna plasmid dna into. Hi, i want to join plpo and plko tables, but i dont see the relation between them. Rnai consortium trc lentiviral shrna horizon discovery. The electronic version of this article in portable document format pdf in a work with an issn or isbn will represent a published work according to the international code of nomenclature for. Manual for the establishment of lacoiptg controlled shrna. For a particular group, file plko contains 10 entries for various group counters. Use text editor or plasmid mapping software to view sequence. Here we would like to draw your attention to plko table in sap. The vector is regarded as a biosafety level 2 material and safe to use due to its modified features deletion of a number of accessory genes implicated in the virulence of hiv, minimal genome of the viral particles, nonreplicating and selfinactivation features, making it incapable of. Plko is a standard sap table which is used to store task list header data and is available within r3 sap systems depending on the version and release level.
It contains all the necessary ciselements for packaging, reverse transcription, and integration for subsequent. Put all 19mers predesigned and newly designed into one. Agei, ecori and ageiecori double digest agei ecori ageiecori 0. This section describes the protocol on how to clone a desired shrna into. Pdf is an electronic document format designed by adobe systems using some language features postscript.
Mtr4 shrna target sequence is ggaaggatttccgatggattt. I can send you a protocol if you decide to go this route. Unlike xhoi, psti restriction sites are absent in plko. The official program for viewing documents in this format, adobe reader. Use with snapgene software or the free viewer to visualize additional data and align other sequences. View notes getting started with shrna in the pgipz lentiviral vector from mathy 677567 at jomo kenyatta university of agriculture and technology, nairobi.
As we know it is being mainly used with the sap ppbd basic data pp component. Novartis developmental and molecular pathways, cambridge, ma, usa. L of ligation mix into competent bacteria with your usual transformation protocol. Here is the title and other details of the protocol. My problem is that i just need to get some operations from plpo, the ones corresponding to certain group. Plko table details plko is a sap table coming under crm module and bbpcrm component. Plasmid sequence and snapgene enhanced annotations. Foxm1 regulates leukemia stem cell quiescence and survival. We use t4 dna ligase from fermentas, but a ligation method of choice can be used. The vector is regarded as a biosafety level 2 material and safe to use due to its modified features deletion of a number of accessory. For a free vector file viewer, try ape or snapgene viewer. It contains all the necessary ciselements for packaging, reverse transcription, and.
A pdf creator and a pdf converter makes the conversion possible. Protocol for the establishment of lacoiptg controlled shrna. Sap abap table plko task list header sap datasheet the best online sap object repository. The methods to create pdf files explained here are free and easy to use. Search for the genes of interest on the rnai consortium trc portal. How to link between plko and plpo for routine in sap. Plko task list header is a standard table in sap r\3 erp systems. This contains the protocol for lentiviral production in 6 cm plate. Below you can find the technical details of the fields that make up this table. You can use this authorization object to control who accesses. The trc lentiviral shrna library is the result of a collaborative research effort based. If we find more than 1 hit, then eliminate the 19mer.
1304 1020 1395 547 331 343 1176 198 923 56 439 987 1136 883 527 751 1447 781 200 903 1199 155 1001 299 794 61 33 119 293 1015 1031 1147 900 1044 1392